Abstract. Housing and breeding conditions have a significant impact on sheep welfare. Well-designed buildings that ensure an appropriate microclimate, unrestricted access to pastures, proper nutrition, gentle handling, and the opportunity to express natural behaviors are key factors influencing sheep welfare. Play is a crucial indicator of lamb welfare. Frequent play activity reflects positive emotions in the animals, whereas stress can significantly reduce their willingness to engage in play. Positive relationships between humans and animals also play an important role. Interactions with caretakers build trust in sheep and lead to positive emotional responses. Observing sheep behavior is one of the most important methods for assessing their welfare. Regular monitoring of sheep behavior by farmers provides valuable insights into their well-being and needs. This enables the detection of potential problems and the implementation of appropriate preventive measures.
Abstract. The aim of the study was to perform histomorphometric analysis of the sole horn in the thoracic limbs and pelvic limbs of primiparous and multiparous cows. During hoof trimming, samples of the sole horn were collected from the fore and hind legs of 30 (15 primiparous and 15 multiparous) cows of the Holstein-Friesian breed. Once prepared, the histological slides were analysed using Multiscan software. The bovine sole horn was subjected to histomorphometric analysis, taking account of the number of horn tubules per 1.5 mm2, diameter of the horn tubules, and distances between the horn tubules. In both primiparous and multiparous cows, a significantly greater diameter of the horn tubules was observed in the fore legs, 51.60 and 48.62 vs. 42.42 and 39.88 μm. In second and third lactation cows, the number of horn tubules in the sole horn was significantly greater in the fore legs, 31.00 per 1.5 mm2 vs. 22.50 per 1.5 mm2 (pelvic limbs). There were no significant differences in the histological structure of the sole horn between the primiparous and multiparous cows.