Danuta Czernomysy-Furowicz, Jolanta Karakulska, Anna Silecka
Abstract. Abstract. Four hundred milk samples were collected from cows. In 78 samples (19.50%) an increase of somatic cells was detected employing the California Mastitis Test (CMT). Therefore, those milk samples were plated (0.1 mL) onto bacteriological and mycological media. Cultures were incubated at 37°C and 20°C for 24h to 72h. Biochemical features of bacteria and fungi were determined in API tests and/or on liquid O-F Medium. The presence of coagulase in Staphylococcus spp. was investigated by tube test using rabbit plasma. For identification of C. albicans the germ tubes test was conducted. Based on microbiological investigation and increase of somatic cells, mastitis was confirmed in all cows, whose milk was selected for further research. The presence of bacteria was confirmed in 64.10% of samples, bacteria and yeast in 34.62% and yeast alone in 1.28%. Sixteen bacterial species were isolated: Staphylococcus spp. (5 species), Enterobacteriaceae (5 species), Streptococcus spp. (4 species), Bacillus cereus and Pseudomonas aeruginosa. Among yeasts, 15 species were identified: Candida spp. (9 species), Blastoschizomyces capitatum, Dipodascus ingens, Geotrichum candidum, Trichosporon asahii, Saccharomyces cerevisiae, Torulopsis conglobata. The most frequent isolated yeasts were Candida spp., mainly C. guilliermondii (6 strains), C. kefyr (4 strains) and C. butyri (4 strains). The most frequent isolated bacteria were Staphylococcus spp. (37 strains), Streptococcus spp. (28 strains) and Enterobacteriaceae (23 strains).
Abstract. Abstract. The research included male goat kids of the white improved breed slaughtered at 90 and 150 days of age. It the tissue composition of fatness after slaughter was qualified was and the haunch as well as the adductor’s chemical composition will pretend the composition of fatty acids and the content of cholesterol. The age category of animals significantly, although only slightly in many cases, influenced the examined meat quality parameters. The influence of age was affirmed on profile of fatty acids in intramuscular fat.
Jolanta Karakulska, Katarzyna Kopron, Paweł Nawrotek, Danuta Czernomysy-Furowicz
Abstract. Abstract. The aim of this study was to define the usefulness of phenotypic determinants used in routine diagnostics and selected genotypic markers for identification and pathogenicity designation of Salmonella spp. strains isolated from animals. Five strains isolated from internal organs of pigs, pigeons and chinchillas, in which salmonellosis was initially recognized, were analyzed. Conventional diagnostic media were used for isolation and differentiation of Salmonella spp. strains. Serological identification of these strains was done using latex test and slide agglutination method. Three swine isolates were classified as Salmonella enterica subsp. enterica serovar Choleraesuis (6.7:c:1.5. serological group C1) and two strains isolated from pigeons and chinchillas as Salmonella enterica subsp. enterica serovar Enteritidis (9:g.m:-, serological group D). Biochemical features of the strains were analyzed by means of API test and differential media. Differences in biochemical activity to six substrata between Salmonella Choleraesuis and Salmonella Enteritidis strains were indicated. Basing on ability to hydrogen sulphide production, two S. Choleraesuis strains were classified as Salmonella Choleraesuis var. america. The susceptibility to 20 antimicrobial agents was carried out by disk diffusion method. Strains revealed resistance to carbenicillin and cefuroxime, variable susceptibility to amoxicillin and gentamicin and phenotypic susceptibility to all other investigated antimicrobials. The DNA rep. ori. gene, the specific marker of genus Salmonella, as well as stn, stpA and spaO genes that determine production of enterotoxin, cytotoxin and invasine respectively, were identified in all strains using PCR.
Abstract. Abstract. The aim of this study was the analysis of kidney factor index (KFI) in roebuck before period procreation. The samples were kidneys and antlers from roebuck hunting in may 2004 on the area of the Rudnik Forest District of the Lublin Regional Directorate of State Forests. After preparation the kidneys were weighting with and without around kidney fat with 1 g exactness. In the other site we were weighting the body roebucks without the had (with 1 kg exactness) and the antlers with cranium (with 1 kg exactness). On this results we calculated kidney factor index (KFI) and correlation between body weight and value KFI. Our investigation shows the connection between body mass and animal condition. The very interesting conclusion was not correlation between roebuck age and individual condition. The value of correlation coefficient between antlers weight and KFI shows that quality of the trophy (roebuck antlers) more depends from personal conditions than body weight.
Olga Szeleszczuk, Bartosz Sipczyński, Piotr Niedbała
Abstract. Abstract. The aim of study was the trial of deep conservation of breeding raccoon dog semen by use of EDTA diluent of different glycerol concentration. The semen was collected by manual method after earlier males accustomed to the room and the person who collected semen. All collected ejaculates were estimated macro- and microscopically. For further steps of dilution, equilibration and freezing ejaculates of volume above 0.3 cm, mobility of 40–60% of spermatozoa of progressive motion were qualified. Modified EDTA diluent was applied of the content 4% (RI), 6% (RII) and 10% (RIII) of glycerol as cryogenic factor. The process freezing and thawing of semen was controlled by mobility estimation as well as morphology of spermatozoa. In plasma of fresh and defrosted semen AspAT and acrosine activity was tested. From 66 collected ejaculates only 30 were qualified for freezing, what was 45.45% of all ejaculates. Sperm mobility in fresh semen was 50% average, but after defrosting participation of a mobile spermatozoa decreased even up to 9.44% in semen with diluent RI with 4% glycerol addition. In this diluent participation of damaged spermatozoa increased from 35.5% to 53%. AspAT activity in fresh semen was 161.38 μIU·ml–1 average. After thawing, activity of the enzyme increased to 232 μIU·ml–1 in RI, 372 μIU·ml–1 in RII and only up to 170 μIU·ml–1 in semen with diluent with 10% glycerol addition (RIII). Similar correlations were observed in acrosine activity.