Research Article

Impact of probiotics on numbers of lactic acid rods producing hydrogen peroxide isolated from mouths of dairy cattle

Marek Selwet, Mariola Galbas, Filip Porzucek, Małgorzata Pociejowska, Tomasz Cłapa

1. Acta Sci. Pol. Zootechnica, 12(3), 2013, 69‒78

Abstract. The investigations were conducted on 60 Holstein-Friesian dairy cows (at age 3 year and weight 590 kg) kept in tie-stall barn. The animals were divided into 3 groups of 20 heads each. The control group (K) was fed diets without probiotics, group (EM) -- was fed diet with the addition of EM probiotic (dose of 150 ml ⋅ t^-1 TMR) and group (T) -- was fed diet with the addition of ToyoCerin probiotic (dose of 0.2 kg ⋅ t^-1 TMR). The volume of 2--10 cm$^3$ saliva was collected from each animal in which the following parameters were determined: number of lactic acid rods from the Lactobacillus genus, number of rods capable of producing hydrogen peroxide. For purpose of precise diagnostics, lactic acid rods were identified on the basis of biochemical traits employing API 50 CHL (BioMérieux), while those manufacturing H2O2 were additionally tested using PCR method. The occurrence of Lactobacillus spp. rods was confirmed in all the examined individuals and in each and every experimental combination. Lactobacillus spp. rods capable of produce hydrogen peroxide were isolated in 17 cows in group K, in 3 individuals in group EM and in 13 animals in group T. EM probiotic strongly significantly restrict the development of Lactobacillus spp. strains are capable to produce hydrogen peroxide.

Keywords: hydrogen peroxide, Lactobacillus spp., probiotics