Research Article

Tolerance of environmental bacteria to heavy metals

Xymena Stachurska , Bartosz Środa, Kamila Dubrowska, Joanna Jabłońska , Marta Roszak , Jolanta Karakulska  

Department of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Piastów 45, 70-311 Szczecin, Poland

Abstract. The aim of this study was to isolate microorganisms with bioremediation potential from soil contaminated with heavy metal compounds, and to analyze the tolerance of these microorganisms to various heavy metal concentrations. In total, 7 bacterial strains were isolated and cultivated. The sensitivity of microorganisms to heavy metals proved to be dependent on the type of medium. The use of liquid media during the 8-hour and 24-hour toxicity tests resulted in reduced tolerance of isolates to the concentrations of selective factors used, relative to culture on solid substrates. In addition, optical density increases in the bacterial cultures have been observed at the highest concentrations of some heavy metals. Briefly, during the 48-hours test 3 strains showed increased optical density values in the presence of 1 mM copper sulfate and 2 strains displayed this ability also in the presence of 1 mM cadmium sulfate and lead nitrate.

Keywords: autochthonous bacteria, bioremediation, soil, heavy metals, toxicity

 

This Article

Received: 14 Apr 2020

Accepted: 15 Jun 2020

Published online: 3 Sep 2020

Accesses: 66

How to cite

Stachurska, X., Środa, B., Dubrowska, K., Jabłońska, J., Roszak, M., Karakulska, J., (2020). Tolerance of environmental bacteria to heavy metals. Acta Sci. Pol. Zootechnica, 19(2), 63–74. DOI: 10.21005/asp.2020.19.2.08.