Research Article

Effectiveness bacteriolysis of Shiga toxin-producing Escherichia coli O157: H7 caused by specific bacteriophage isolated from pig slurry

Bartłomiej Grygorcewicz, Magdalena Struk, Agata Wasak, Paweł Nawrotek

West Pomeranian University of Technology, Szczecin, Poland

Abstract. This study was aimed to in vitro evaluation of a bacteriophage isolated from pig slurry samples as a bacteriolytic agent to elimination Shiga toxin–producing E. coli (STEC) O157: H7. Collected STEC O157: H7 strains was susceptible for bacteriophage infection. The phage infection at the multiplicity of infection (MOI) of 1, 5 or 8 caused a rapid cell lysis resulting in lack of growth in the cell concentration. In conclusion, the data obtained from the present study shows that bacteriophage isolated from pig slurry is a lytic bacteriophage capable to killing strains of E. coli O157: H7. Our results demonstrate that specific bacteriophages have the potential to biocontrol STEC strains in environment.

Keywords: Key words: STEC, E. coli O157: H7, bacteriophages, biocontrol, pig slurry

 

This Article

Accepted: 24 Mar 2015

Published online: 30 Apr 2017

Accesses: 450

How to cite

Grygorcewicz, B., Struk, M., Wasak, A., Nawrotek, P., (2015). Effectiveness bacteriolysis of Shiga toxin-producing Escherichia coli O157: H7 caused by specific bacteriophage isolated from pig slurry. Acta Sci. Pol. Zootechnica, 14(1), 69–76.