Research Article

Analysis of indel polymorphism of the PRNP gene in water buffalo, Bubalus bubalis

Paulina Kobak, Piotr Sablik, Agata Żukiewicz, Andrzej Syczewski, Wiesław Lechowicz

1. Acta Sci. Pol. Zootechnica, 13(1), 2014, 51‒56

Abstract. The aim of this study was to examine insertion-deletion polymorphisms (one being 12bp-long in intron 1 and second being 23bp-long in the promoter) in water buffalo. The blood samples were collected from two water buffalo herds (40 individuals). DNA was isolated using a commercial Master Pure DNA Purification Kit. After conducting 2 PCRs, and then electrophoreses in 4% agarose gel, it was found that there was no polymorphism in either PRNP 12 ins/del the PRNP 23 ins/del in the analyzed group of animals. All individuals were characterized by the ins/ins genotype for both polymorphisms. Because of the fact that four different genotypes were found in the Anatolian breed and only one genotype was identified in the breed examined in the present study, more breeds of this species should be included in further research.

Keywords: PRNP gene, DNA polymorphism, prion disease, water buffalo