Research Article

SERUM ELECTROPHORETIC ANALYSIS OF CLINICALLY HEALTHY HORSES USING TWO TYPES OF AGAROSE CARRIER

Anna Silecka, Danuta Czernomysy-Furowicz, Karol Fijałkowski

1. Acta Sci. Pol. Zootechnica, 7(2), 2008, 23‒30

Abstract. The electrophoresis of 14 sera taken from clinically healthy Standardbred horses was preformed by means of two types of agarose gel. Eight fractions were obtained with Protein Gel 100 (Cormay). The mean concentration for each fraction was as follows: albumin – 30.45 g·L^–1, a₁ – 2.65 g·L^–1, a_2a – 4.47 g·L^–1, a_2b – 3.04 g·L^–1, a_2c – 6.76 g·L^–1, b₁ – 8.68 g·L^–1, b₂ – 3.15 g·L^–1, g – 9.43 g·L^–1. Whereas, using Hydrogel HR nine fractions were acquired, whose mean concentrations were: albumin – 31.34 g·L^–1, a₁ – 2.10 g·L^–1, a_2a – 2.34 g·L^–1, (consisting of: a_2aa – 0.95 g·L^–1 and a_2ab –1.47 g·L^–1), a_2b – 5.07 g·L^–1, a_2c – 4.49 g·L^–1, b₁ – 7.30 g·L^–1, b₂ – 3.85 g·L^–1, g – 10.06 g·L^–1. In order to measure total protein concentration (mean value: 68.61 ± 11.07 g·L^–1), Lowry’s method, adjusted to microplace reader Elx800 (Bio-Tek Instruments INC., Universal Microplate Reader), was employed. Albumin/ globulin ratio equals 0.80 g·L^–1 and 0.84 g·L^–1, respectively. Furthermore, a 1– antitrypsin localization in proteinogram was determined by the immunofixation kit (GEL IFE Cormay) and Polyclonal Rabbit Anti-Human Alpha – 1 Antitrypsin (DakoCytomation).

Keywords: albumin, electrophoresis, globulin, proteinogram, serum fraction, total protein